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Sains Malaysiana 53(6)(2024): 1343-1362
http://doi.org/10.17576/jsm-2024-5306-10
Design
and Characterisation of Curcumin-Loaded Nanostructured Lipid Carriers for Safe
and Efficient Topical Drug Delivery in The Treatment of Pressure Ulcer
(Reka
Bentuk dan Pencirian Pembawa Lipid Berstruktur Nano Berpengisi Kurkumin untuk
Penghantaran Ubat Topikal yang Selamat dan Berkesan dalam Rawatan Ulser
Tekanan)
NUR AMIRA MOHD SHAMSUDDIN1,
FAZREN AZMI1, KHAIRANA HUSAIN2, LEONG LEK MUN3 & MOHD HANIF ZULFAKAR1,*
1Centre for Drug Delivery Technology &
Vaccine, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, 50300 Kuala
Lumpur, Malaysia
2Centre for Drug and Herbal Development, Faculty
of Pharmacy, Universiti Kebangsaan Malaysia, 50300 Kuala Lumpur, Malaysia
3Prima Nexus Sdn. Bhd., 28-1B, Jalan Puteri 1/2,
Bandar Puteri, 47100 Puchong, Selangor, Malaysia
Received: 14 February 2024/Accepted: 16 May 2024
Abstract
Pressure ulcers (PU) are chronic lesions with a high prevalence in at-risk populations. Although curcumin has various therapeutic properties, its use as a dermal therapeutic agent is limited by its poor solubility and permeability. This study aimed to develop nanostructured lipid carriers (NLCs) to facilitate the topical use of curcumin as wound healing agent in treating PU. The optimised curcumin-NLCs were characterised for their physicochemical properties, drug release, and skin permeation, while effectiveness and toxicity were investigated via cell viability, scratch assays, and an in vivo skin irritation test. The optimised curcumin-NLCs comprised 3.5% solid lipids, 1% surfactant, and 2% co-surfactant with an average particle size of 134.68±6.0 nm, polydispersity index of 0.248±0.00, zeta potential of −44.94±5.44, percentage of encapsulation efficiency of 93.42±0.32%, and drug loading of 0.11±0.00%. Curcumin-NLCs showed stable physicochemical properties and extended drug release for up to 48 h, with a cumulative release of 77.72% following a first-order kinetics. The formulation exhibited slower curcumin permeation than the free curcumin. Furthermore, the safe and non-toxic curcumin-NLCs were highly effective at 0.98 μM and showed a high rate of wound closure without skin irritation. Collectively, the NLCs developed for topical delivery of curcumin demonstrated prolonged and sustained release and improved wound healing activity, which indicates that curcumin-NLCs are a viable strategy for treating PU.
Keywords: Curcumin; nanostructured
lipid carrier; physicochemical characterisation; pressure ulcers; toxicity
Abstrak
Kata kunci: Ketoksikan; kurkumin;
pembawa lipid berstruktur nano; pencirian fizikokimia, ulser tekanan
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*Corresponding author; email: email: hanifzulfakar@ukm.edu.my
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